To investigate expression of VEGF isoforms in our mouse fibrosarcoma cell lines, their corresponding solid tumour extracts and normal mouse and human cells/tissues, we adopted a PCR approach using primers designed to amplify VEGFxxx and VEGFxxxb families simultaneously, consistent with the primer design strategy previously reported for the identification of human VEGF165b [26]. The gene discussed is VEGFA; the disease is fibrosarcoma.