Importantly, the drug had no significant effect on the severity of ischemia following ET-1 (perfusion reduced to 7.5 ± 5% of baseline for KB-R7943 vs. 7.7 ± 4% of baseline for vehicle; P > 0.05; Fig. 3D & F), showing that the protective effect of KB-R7943 on axonal conduction was not due to a diminution of the severity of ischemia. Here, EDN1 is linked to ischemia.