Even the complete absence of DNMT3B in 3BKO cells did not affect the hypermethylation phenotype of HCT-116 colon cancer cells, indicating that the observed antiproliferative effects of DNMT3B depletion are not caused by DNA demethylation of putative DNMT3B target genes. This evidence concerns the gene DNMT3B and malignant colon neoplasm.