To investigate the role of securin in the anticancer effects of BPR0L075, several human cancer cells with different securin expression levels, including lung cancer cells (A549), human breast cells (MCF-7 and MDA-MB-231), melanoma (MDA-MB-435) and colorectal cancer cells (HCT116), were used (Fig. 1A). Here, PTTG1 is linked to melanoma.