BRAF and neoplasm: ASLNAqPCR quantification of the mutated to wild type allele ratio clearly indicated the presence of tumor cell subclones in 7 of the 16 discrepant KRAS results (cases 1, 8, 9, 10, 11, 12, 16 of Table 7, Figure 6, panels C and D) and in 3 of the 5 discrepant BRAF results (cases 2, 3, 4 of Table 8).