Thus, a single marker may not be sufficient to isolate CSCs, and as a result, several markers are employed together to isolate certain types of CSCs, such as CD44+CD133+CD24+ cancer stem-like cells involved in murine melanoma [42], [43], CD133+CD34+ melanoma CSCs [44], and CD133+/nestin+ lung cancer CSCs [45]. This evidence concerns the gene NES and lung cancer.