Since we have found that Ang II-mediated cardiac hypertrophy depends on the expression of PLZF, and that PLZF activation is driven by AT2 in the heart [15]; and since GATA4 is one of the well known factors involved in cardiac development and hypertrophy [26], we examined the possible transcriptional role of PLZF on GATA4 gene expression by luciferase assay in R3T3 cells which express the AT2 receptor but not AT1. The gene discussed is GATA4; the disease is cardiac hypertrophy.