SUN2 and Emery-Dreifuss muscular dystrophy: Immunoblot analysis of control and patient fibroblasts with the SUN2 specific mAb K80-207-11 confirmed the results obtained for the mRNA at the protein level revealing a decrease in EDMD/CMT cell lysates at higher passages (passage 6 vs passage 8 or 16, respectively) (Fig. 4c, d).