To analyze Akt/PLCγ expression, proliferation capacity, and cell migration we cultured different tumor cell lines (MDA-MB-468 breast and SW480 colon cancer cells) at physiological (5.5 mM, control) and diabetogenic (11 mM) glucose concentrations without and with 100 ng/ml insulin. The gene discussed is AKT1; the disease is malignant colon neoplasm.