The crosstalk between calcitriol/Vdr and Hh pathways prompted us to investigate the effect of calcitriol on Vdr and Hh signaling, growth, apoptosis, and differentiation of Hh-associated RMS in vitro and in vivo using the Ptch neo67/+ mouse model for ERMS [12]. This evidence concerns the gene VDR and embryonal rhabdomyosarcoma.