To determine the concentration of bevacizumab needed for neutralizing the secreted VEGF by bone metastatic prostate cancer C4-2B cell line, ELISAs were performed to measure the levels of VEGF in conditioned media in C4-2B and C4-2B co-cultured with microvessel cells under bevacizumab or control IgG treatment. Here, VEGFA is linked to metastatic prostate carcinoma.