We found that whereas the spot volume of ubiquitin in the 2-D electrophoresis gels (Figure 8A) and monoubiquitin as determined by Western blot analysis (Figure 8B) were significantly elevated in RVLM in our experimental endotoxemia model, the mRNA level of ubiquitin remained constant (Figure 8C). The gene discussed is UBB; the disease is serum lipopolysaccharide activity.