In addition, the analysis of IFN-γ production by ELISPOT of CD8 T cells from Chagas' disease patients showed that the frequency of IFN-γ producing-CD8 T cells is very low among those patients suffering the most severe form of the disease, and among individuals living in areas of active transmission of the disease, indicating that severe Chagas' cardiomyopathy could be related with the frequency of IFN-γ – producing T cells [31], [35]. The gene discussed is IFNG; the disease is Chagas disease.