In this study, we provide in vivo evidence for anti-tumor activity linked to anti-angiogenesis by HSA/TIMP-2 and further clarify that the anti-angiogenesis is associated with attenuated MMP-2 expression independent of MMP-2 activity as demonstrated by the absence of effects on MT1-MMP expression and pro-MMP-2 activation. This evidence concerns the gene TIMP2 and neoplasm.