SPP1 and non-small cell lung carcinoma: We constructed tissue microarray, and used immunohistochemical method to assess the expression of 10 bone metastasis-related tumor markers in primary NSCLC tissue, which involved multi-step process of bone metastasis [3], including the proliferation, adhesion, escape (MMPs, OPN, c-Src) of primary tumors; targeted metastasis to bone (CXCR4); bone-specific adhesion and implantation (BSP); formation of metastases in bone (IGF1R, BMPs, PTHrP) and metastasis-associated cell signaling pathways (PI3K, NFκB).