The amount of methylated GSTP1 promoter was determined for each segment of BS-converted DNA by MSP assays in duplicate, and the receiver operating characteristic (ROC) curves were constructed accordingly to evaluate the performance of the mGSTP1 promoter as a biomarker to distinguish HCC from non-HCC including hepatitis and cirrhosis (Fig. 3B). This evidence concerns the gene GSTP1 and hepatitis A virus infection.