Highly metastatic human breast cancer cells D3H2LN (an in vivo-selected subclone of MDA-MB-231 cells expressing luciferase [22]) were exposed to AngII (100 nM) for 24 hrs (or vehicle for control group) and injected intra-cardiacally into the bloodstream of nude mice in order to recapitulate the late and essential steps of cancer metastasis, i.e. extravasation and colonization [22], [23]. The gene discussed is AGT; the disease is cancer.