Consistent with a role for AMPK both in early events during viral replication and in spread as measured by plaque assay Figure 1A), we observed an increase in viral infection at early time points before virus spread, as well as increased spread in cells lacking AMPK by monitoring the production of RVFV N protein over time by microscopy (Figure S1A–B). The gene discussed is PRKAA2; the disease is viral infectious disease.