Transduction of various cancer cell lines with AAV-TRE-TRAIL&AAV-Tet-On under the presence of Dox resulted in significant cell death by apoptosis in SMMC-7721, SMMC-7402 liver cancer cells, and A549 lung cancer cells, but not in HepG2 liver cancer cells (Figure 2A). Here, TNFSF10 is linked to liver cancer.