As shown in Figure 5, rAAV-TRE-TRAIL&rAAV-Tet-On administration and Dox induction resulted in the cleavage of procaspase-3 and procaspase-8, but not in the animals without Dox in the water, indicating that Tet-On system-controlled sTRAIL expression in the animals induces tumor cell death by apoptosis via the classic apoptosis pathway characterized by caspase activation. This evidence concerns the gene TNFSF10 and neoplasm.