In the current work, we develop and demonstrate an efficient therapeutic strategy model against a Photynus pyralis luciferase (Pluc) and enhanced green protein (eGFP) expressing U87 glioblastoma model in SCID mice, using hAMSCs genetically modified to express a tri-functional reporter comprising Renilla reniformis luciferase (Rluc), red fluorescent protein (RFP) and a truncated version of HSV-TK (tTK). This evidence concerns the gene TTK and glioblastoma.