IGHG3 and Chagas disease: Figure 4 shows that there were no differences in the distribution of IgG subclasses between groups, with IgG subclass levels ranking from IgG1 > IgG3 > IgG2 > IgG4 in both HLA+ and HLA− subjects. We further stratified the subjects in our study separating them into the two clinical forms of Chagas disease, CCC (n = 32) and ASY (n = 39), inside the two HLA groups (HLA+ and HLA−), but no significant difference was observed (Figure 5).