In order to test whether SRY could function on this reporter construct, the reporter construct was cotransfected with either an SRY expression plasmid (pCXN-2-SRY-3HA) or an empty plasmid (pCXN-2) into human prostate adenocarcinoma LNCaP cells, which have very low expression levels of endogenous SRY, and relative luciferase activity was analyzed. The gene discussed is SRY; the disease is prostate adenocarcinoma.