Relevant to this, 4 of the 8 OVA-treated mice had high levels of IL-1β and TNF-α in their serum while the other 4 mice (like all of the control mice) had very low levels of both cytokines, despite all 8 OVA-treated mice having high levels of airway inflammation as evidenced by eosinophilia in BALF and tissues as well as epithelial swelling and cell death. Here, TNF is linked to Increased total eosinophil count.