Western blot analysis (Fig. 3A,B) showed a reduction of the immunoreactivity of synaptophysin (−23.3±4.9%, n = 6, P<0.05) and SNAP25 (−28.6±6.5%, n = 6, P<0.05) in hippocampal membranes, indicating the occurrence of synaptic degeneration, previously reported to occur in models of type 1 diabetes [14], [15]. This evidence concerns the gene SYP and type 1 diabetes mellitus.