Infection with Ad.DD3.D55-PTEN increased the number of cells in the sub-G1 phase in the tested prostate cell lines(CL1, DU145, 22RV1, PC3) and a higher apoptotic index was found in 22RV1 cells (43.135%) than in DU145, PC3 or CL1 cells, consistent with the higher expression of E1A in the 22RV1 cell line(Fig. 4B). The gene discussed is DHTKD1; the disease is infection.