The oncolytic virus Ad.DD3.D55 and Ad.DD3.D55-PTEN led to an increase in phospho-Akt expression at 24 h after infection(Fig. 2.D); the activation of PI3K-Akt signaling is the pathway employed by viruses for viral endocytosis and replication [34], [35], [36], [37]. Here, PTEN is linked to infection.