The antitumor efficacy of PTEN in the oncolytic virus was much enhanced compared with the adenovirus-mediated PTEN that was previously reported for use in prostate cancer [48], [52], it could be resulted from that Ad.DD3.D55-PTEN induced a massive apoptosis in prostate cancer cells by intrinsic and extrinsic pathways. Here, PTEN is linked to prostate carcinoma.