As shown in Figure 4A, the samples containing NKG2D-Fc and NKG2D-Fc-IL2 produced shifts significantly different from the TC-1 tumor cells treated with Con-Fc or Con-Fc-IL2, demonstrating that intramuscular injection of a DNA construct encoding the proteins consisting of NKG2D-Fc followed by electroporation can produce detectable amounts of functional chimeric protein in the serum. Here, IL2 is linked to neoplasm.