To determine if the purified NKG2D-Fc could bind to murine NKG2D ligand-expressing tumor cells, we selected three NKG2D ligand-expressing tumor cell lines (TC-1, MOSEC, and OVHM; all express Rae-1, a murine NKG2D ligand) and one Rae-1-negative tumor cell line (B16F10). The gene discussed is KLRK1; the disease is neoplasm.