In healthy control subjects the level of RAGE expression increased from 3.8±0.5% to 31.7±4.6% of CD4+ (p = 0.01) and from 4.7±0.85% to 34.8±6.79% (p = 0.03) on CD8+ T cells following culture with anti-CD3 Ab, whereas it decreased from 28.0±3.8% to 11.3±4.8% (p<0.05) on CD4+ and 29.4±3.5% and 13.7±5.9% on CD8+ T cells in patients with T1D (p<0.05) (Figure 6). This evidence concerns the gene CD4 and type 1 diabetes mellitus.