In this work, Nod1−/− mice were shown to be very susceptible to T. cruzi, succumbing to the infection and displaying higher parasitemia and parasite loads in the spleen and heart tissues, although NOD1 deficiency does not impair the production of different cytokines as IL-12, TNF-α, IFN-γ, or IL-10. The gene discussed is TNF; the disease is parasitic infectious disease.