Thus, we inspected the gene expression levels of the examined genes (CD4, CD25, FOXP3, TGF-β, IFN-γ, IL-10, CTLA-4, PD-1, PD-L1, ARG1) in the CRC patients and confirmed they were all upregulated in the cancer patients (Table 3). This evidence concerns the gene FOXP3 and colorectal carcinoma.