Our semiquantification analysis of MCM-2 labeling index in the three epithelium layers, did not reveal immunoreactive stained cells for control as well for LGCIN in the superficial layer, however on HGCIN, positive stained cells was seen (range 0–56), in both HIV positive and negative cervices suggesting that MCM-2 probably may have a role to distinguish LG-CIN from HG-CIN. This evidence concerns the gene MCM2 and cervical squamous intraepithelial neoplasia.