Elevated AAb levels to at least one of the original six antigens in the EarlyCDT-Lung test (p53, CAGE, NY-ESO-1, GBU4-5, Annexin I, and SOX2-N), using the original published cutoffs, gave an overall sensitivity for lung cancer detection of 39 % with an unadjusted specificity of 89 %, while elevated levels of AAbs to at least one of eight antigens tested (p53, CAGE, NY-ESO-1, GBU4-5, HuD, Annexin I, MAGE A4, and either SOX2-N or SOX2-B) with the new and optimized cutoffs gave an overall sensitivity for lung cancer detection of 42 % with an unadjusted specificity of 91 %. Here, SOX2 is linked to lung cancer.