More recently, Moelans et al. evaluated the usefulness of MLPA assay for the simultaneous testing of HER-2/neu and TopoII alpha gene amplification status in 353 paraffin-embedded breast cancer samples, since TopoII amplification status determines the anthracycline sensitivity, showing TopoII alpha amplification in 9% and HER2 in 13% of patients, respectively. This evidence concerns the gene ERBB2 and breast cancer.