In the present study we investigated the migratory activity of the two hybrid cell lines M13MDA435-1 and M13MDA435-3 in dependence of EGF and SDF-1α stimulation in comparison to M13SV1-EGFP-Neo breast epithelial cells exhibiting stem cell characteristics and HS578T-Hyg breast cancer cells. The gene discussed is EGF; the disease is breast cancer.