In order to compare the expression level of Notch1 gene in different T-ALL samples with Notch1 mutations or with wild-type (WT) Notch1 status, the expression level of Notch1 gene was analyzed by real-time PCR, in comparison with healthy controls (0.37 ± 0.33), significantly higher expression of Notch1 was found in both T-ALL with WT Notch1 (6.34 ± 7.03) (p = 0.0006) or with Notch1 mutations (Mut Notch1) (20.47 ± 10.81) (p < 0.0001) (Figure 3). Here, NOTCH1 is linked to acute lymphoblastic leukemia.