TP53 and Hydrocephalus: However, reduction in the size of the eye and hydrocephaly was consistently observed at 48 hpf in embryos co-injected with p53-atgMO (Fig. 2B, middle row, right panel), and a statistically significant reduction in eye size was seen at 48 and 72 hpf (Fig. 2D), confirming that these changes are bona fide effects of reduced bbs9 function.