Further, the mineralization activity of the osteoblasts was also significantly impaired (p<0.05) when the differentiation was elicited for 2 weeks in presence of conditioned medium from cancer cells that were silenced for GLI1 (Figure 5C), suggesting that active Hh signaling in the tumor cells is vital to their ability to induce osteoblast differentiation. The gene discussed is GLI1; the disease is neoplasm.