IHH and ductal breast carcinoma in situ: While the staining intensity of IHH was comparable (p>0.05) in normal tissues and in tissues derived from Ductal Carcinoma In Situ (DCIS), the tissues derived from invasive cancer (representing Infiltrating Ductal Carcinoma Grades II–IV) and from metastatic breast cancer exhibited significantly (p<0.0001) increased staining intensity for IHH (Figure 1A; images a and b).