However, the way we have studied aTregs appears accurate also in respect to current knowledge, particularly since recent literature makes it more and more likely that CD25 (gated with a high threshold) is a more important marker for active Tregs than Foxp3: it has been well demonstrated that a relevant subset of peripheral human CD4+Foxp3+ cells that is increased in SLE and most characteristically shows low or absent (but not bright) surface CD25, does not consist of functionally active Tregs [11], [25]–[28], [53], [54]. The gene discussed is CD4; the disease is systemic lupus erythematosus.