Pyrosequencing validation was performed on 2 DMRs discriminating between AML and NBM (DPP6, SPHKAP), 2 DMRs that were hypermethylated in 2 AML subtypes; ST6GAL2 in t(8;21), HHEX in trisomy 8 AML and an AluJb repeat that was differentially hypomethylated in t(8;21) AML. This evidence concerns the gene DPP6 and acute myeloid leukemia.