Thus, MDA-MB-231 breast cancer cells, which express low levels of endogenous EBP50, were transfected with an EBP50 expression plasmid to overexpress EBP50, and MCF-7 breast cancer cells, which express high levels of endogenous EBP50, were transfected with an EBP-RNAi plasmid to knock down its expression. The gene discussed is NHERF1; the disease is breast cancer.