We found that increased levels of DACT1 led to higher levels of membrane-associated β-catenin, but the E-cadherin expression pattern in colon cancer cells was unaltered by DACT1 siRNA or the overexpression of DACT1. We also found DACT1 enhanced anchorage independence, as well as the migratory and invasive potential of colon cancer cell lines. This evidence concerns the gene CDH1 and colonic neoplasm.