Follow up analyses of these compounds revealed their capacity to: block β-catenin binding to GST-Tcf4 in vivo; reduce expression of a Wnt/β-catenin luciferase reporter; restore the β-catenin induced axis duplication of Xenopus embryos when co-injected with β-catenin; inhibit expression of the Wnt target genes Myc and CyclinD1; and retard the proliferation of colon cancer cell lines known to display hyperactive Wnt signaling in vitro[45]. This evidence concerns the gene CCND1 and colonic neoplasm.