IFNG and influenza: Assessment of multiple cytokine production in the culture supernatant further showed that priming with influenza-LACK158–173 induced Th1 responses as the LACK158–173 stimulated T cells produced IFN-γ and IL-12, but not IL-4 or IL-10 (very low levels of IL-10 were detected following PR8-LACKins most likely due to influenza itself, which has been shown to induce IL-10 [27]) (Fig. 2).