VPS52 and infection: Mice infected with the double gat1/are1Δ tar1Δ mutant also succumbed to infection slightly faster than mice infected with the wild-type strain, consistent with our previous work demonstrating that Gat1/Are1 modestly represses virulence during murine infection (between 15 and 22 days post infection, median survival of 19 days) (WT vs gat1/are1Δ tar1Δ, P = 0.0342) [34].