APOH and autoimmune polyendocrinopathy: It was also showed that when female BALB/c mice were immunized once with β2-GPI in complete Freund's adjuvant (CFA) or with CFA alone (controls), the APS mice develop elevated levels of antibodies against negatively charged phospholipids and β2-GPI, accompanied by neurological impairment consisting of both cognitive and behavioral changes [51].