These studies demonstrated that db/db eNOS−/− mice exhibit significant albuminuria and glomerular pathology that parallel the later phase of DN in patients with type 2 diabetes including arteriolar hyalinosis, mesangial expansion, thickening of GBM, and focal segmental and early nodular glomerulosclerosis [25, 41] (Figure 2). This evidence concerns the gene NOS3 and type 2 diabetes mellitus.