FSCN1 and esophageal squamous cell carcinoma: Since Fascin homolog1 (FSCN1) was confirmed to be regulated by miR-145 and miR-143 simultaneously with 3′UTR luciferase reporter assay, the protein levels of FSCN1 in five ESCC cell lines were determined by Western Blotting to explore the actual association between FSCN1 protein and miR-143/miR-145 cluster.