In addition, we performed a quantitative analysis for Tau protein in ischemic cortex over time after transient cerebral ischemia/reperfusion using western blotting and found significant increases in the total Tau and hyperphosphorylated Tau (pS202 and pS199), suggesting that a specific set of kinases were activated during the short period after reperfusion, which may contribute to the formation of intracellular Tau aggregates. The gene discussed is MAPT; the disease is brain ischemia.