In addition, we performed a quantitative analysis for Tau protein in ischemic cortex over time after transient cerebral ischemia/reperfusion using western blotting and found significant increases in the total Tau and hyperphosphorylated Tau (pS202 and pS199), suggesting that a specific set of kinases were activated during the short period after reperfusion, which may contribute to the formation of intracellular Tau aggregates. This evidence concerns the gene MAPT and Cerebral ischemia.