In contrast, Fisher et al. [8] reported that the expression of CYP2E1 significantly decreased with the progression of human nonalcoholic fatty liver disease (NAFLD) from simple fatty liver (steatosis) to the more severe NASH, and the expression of CYP1A2, CYP2C19, CYP2B6 and CYP3A4 also tended to decrease with increasing severity of NAFLD. This evidence concerns the gene CYP3A4 and metabolic dysfunction-associated steatotic liver disease.