To determine whether the effect of BF on C26:0 levels is mediated by activation of either PPARα, PPARβ/δ or PPARγ, X-ALD fibroblasts were incubated with the PPARα ligand WY14643, PPARβ/δ ligand GW501516, or the PPARγ ligand rosiglitazone either alone or in all possible combinations. This evidence concerns the gene PPARD and X-linked adrenoleukodystrophy.