EZH2 and neoplasm: The results from soft agar assay further showed that the treatment of C4-2B cells with 10 or 25 μM BR-DIM reduced the colony size and numbers (Fig. 5C and 5D), suggesting that BR-DIM could eliminate tumor cells especially the cells with CSCs or CSLCs characteristics by up-regulating let-7 family and consequently by down-regulating the expression of EZH2.